Human Immunoglobulins are purified from human plasma/serum with the buffers used being phosphate or tris buffered saline coming with slightly differing salt concentrations. It is available in liquid form. Coming from a diverse class of human antibodies that are made mainly by Immunoglobulin G (comprising is 80% of immunoglobulins in human plasma), here, IgA, IgG, and IgM come characterized for protein content through absorbance at 280nm using appropriate extinction coefficient where purified IgE comes tested using Lowry protein assay. The serum forms of IgE (unpurified) are also tested by EIA. With Immunoglobulins A, D, E and M making up approximately 20% of remaining human immunoglobulins, here each class of antibody gets designation from heavy as well as light peptide chains making up antibody structure.