Usage/Aplication | Laboratory Chemical |
Brand | Recombigen |
Form | Liquid |
Packaging Type | Box |
Shelf Life | 16 Month |
Pack Size | 2X50 ml |
Temperature | 2-8 Deg C |
Country of Origin | Made in India |
Albumin is a liquid reagent kit used for the determination of albumin in serum or plasma based on Bromocresol-Green(BCG) method.
Principle:
Serum albumin in the presence of Bromocresol-Green (BCG) under acidic condition forms a green colored complex whose absorbance read at 630nm wavelength. Absorbance at this wavelength is proportional to the albumin concentration in serum/plasma.
Clinical Significance:
Elevated levels of Serum Alkaline Phosphatase are generally observed in growing children. In grown up adults the elevated levels are associated with born diseases like, metastasis, rickets, Paget’s disease, healing fractures, as wells in liver and biliary tract diseases, ore over progressive increase in serum Alkaline Phosphatase activity maybe the firstindication of adverse reaction to certain therapeutic drugs like androgens, anabolic steroids, estrogen, sulphonamides, MAO inhibitors etc. The decrease levels are found in severe anaemia, scurvy etc.
Principle:
Alkaline Phosphatase in serum catalyze the hydrolysis of p. nitro phenyl phosphate to p. nitro phenol and phosphate. P. nitro phenol is yellow colored compound. As the reaction progresses the rate of absorbance increases which is proportional to the activity of Alkaline Phosphatase in the Sample. This reaction takes place in alkaline medium and in presence of magnesium ions. The change in absorbance is measured at 405 nm.
Additional Information:
- Production Capacity: 1000